I used to, but I do not any longer. Its been determined that this is unnecessary, its all over the Zeovit forum. I let it sit for 30 minutes, letting it brew for 8 hours may increase the quantities of bacteria, but they have said its more of a waste of time. Like I said its tough to follow Zeovit's directions, a lot of people go by trial and error and what works best for them, which is different in every case. I would also always forget after 8 hours and find the zeozym sitting on my counter the next day. LOL!!!
I agree. Even after 8 hours all if it settles in the bottom anyways.