Well the fresh water and peroxide should have handled that. If you want to be through maybe reduce the particulates etc you can remove and flush it. My sand bed is very shallow so it was easy to drain my tank down to the sand bed bleach and sterilize my wet dry vacuum then goto town and suck out all the sand wash it and put it back in
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Freshwater + peroxide is double teaming velvet and ich. Neither can live in it. The only reason that I would remove the sand is if I'm concerned with dead bacteria etc. As you are flushing the tank twice just siphon the sand in the tank and release all that nasty stuff and leave it in. I do not think you need to remove it . I wouldn't.
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I have a 5 Lb. kit of Marco Aquascape Mortar Mix that I never used if you want it.
http://www.marcorocks.com/aquascape-supplies/e-marco-400/
Jim I appreciate that, if I'm down in Mt Laurel at my office I will take you up on it!
Btw you don't happen to work for Flo do you?
Progressive??
Yes Progressive, we have an office in Mt Laurel and the branch manager there is named James, but goes by Jim. Would be funny if you're the same person, but probably not.
The dosing amount of peroxide should be equivalent to the volume. To get around 150 ppm
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What is the procedure to know what ppm you are at?